Israeli company Arkia offers on its website a new route from Tel Aviv to Prague. Regular flights start on October 20 with scheduled departure from Tel Aviv every Thursday and Sunday at 09.40, and return to Tel Aviv at 18.30 after a stop in Prague between 12.30 and 14.00, according to planes.cz. 

From Sunday, September 15, 2019, Vueling began flying to Prague from Florence Mondays, Wednesdays, Fridays and Sundays, More by Prague Airport. More by planes.cz.

During the winter season, Czech Airlines will increase frequencies on flights to Kiev and Moscow. Flights to the capital of Ukraine will now operate up to eight times a week, while Sheremetyevo International Airport in Moscow will be served three times a day, Prague Airport informed. Czech Airlines will also extend several summer routes into the winter season. Its winter schedule will thus feature flights to Beirut and Odessa, which will operate at least two times a week, and a connection to Keflavík, Iceland, now with a stopover in Copenhagen, which will be available to passengers daily.
Moreover, capacity on three direct routes operated by Czech Airlines will be increased. Beginning at the end of October, capacity will be increased on selected flights to Budapest and all flights to Düsseldorf. Smartwings is also increasing the number of flights it operates in its winter schedule. The route between Prague and Rostov-on-Don will now be served three times a week and more flights will be operated on the route to St. Petersburg, which will be available every day from the start of the winter schedule, There is also the plan to relaunched service to Dubai, United Arab Emirates, Prague Airport informed.
 

As of June 1, 2020, Emirates will close the evening flight from Dubai to Prague. More by planes.cz. 

Ryanair announced two new Prague routes to Rhodes and Thessaloniki in Greece, both with a twice weekly service commencing in March 2020 as part of Ryanair’s Czech Republic Summer 2020 schedule, which will be launched shortly, Prague Airport announced.